Composite

Part:BBa_K4614105:Design

Designed by: Zhang Xiyuan   Group: iGEM23_CAU-China   (2023-09-30)


T7-RBS-Wza-SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 704


Design Notes

none


Source

T7 promoter, RBS and T7 terminator are already present on plasmid pET-30a(+). wza was obtained from the genome of Escherichia coli K-12 by PCR amplification. SpyCatcher is obtained through synthesis.

References